Understanding the role of the fructose-1,6-bisphosphatase gene for enhancing the photosynthetic rate in Arabidopsis thaliana.

Transgenic Arabidopsis thaliana (ecotype Columbia) was successfully transformed with the gene fructose-1,6-bisphosphatase (FBPas e) and named as AtFBPase plants. Transgenic plants exhibited stable transformation, integration and significantly higher expressions for the transformed gene. Morphological evaluation of transgenic plants showed increased plant height (35cm), number of leaves (25), chlorophyll contents (28%), water use efficiency (increased from 1.5 to 2.6µmol CO2 µmol-1 H2 O) and stomatal conductance (20%), which all resulted in an enhanced photosynthetic rate (2.7µmolm-2 s-1 ) compared to wild type plants. This study suggests the vital role of FBPase gene in the modification of regulatory pathways to enhance the photosynthetic rate, which can also be utilised for economic crops in future.

Upregulation of TaHSP90A transcripts enhances heat tolerance and increases grain yield in wheat under changing climate conditions.

Plants have certain adaptation mechanisms to combat temperature extremes and fluctuations. The heat shock protein (HSP90A) plays a crucial role in plant defence mechanisms under heat stress. In silico analysis of the eight TaHSP90A transcripts showed diverse structural patterns in terms of intron/exons, domains, motifs and cis elements in the promoter region in wheat. These regions contained cis elements related to hormones, biotic and abiotic stress and development. To validate these findings, two contrasting wheat genotypes E-01 (thermo-tolerant) and SHP-52 (thermo-sensitive) were used to evaluate the expression pattern of three transcripts TraesCS2A02G033700.1, TraesCS5B02G258900.3 and TraesCS5D02G268000.2 in five different tissues at five different temperature regimes. Expression of TraesCS2A02G033700.1 was upregulated (2-fold) in flag leaf tissue after 1 and 4h of heat treatment in E-01. In contrast, SHP-52 showed downregulated expression after 1h of heat treatment. Additionally, it was shown that under heat stress, the increased expression of TaHSP90A led to an increase in grain production. As the molecular mechanism of genes involved in heat tolerance at the reproductive stage is mostly unknown, these results provide new insights into the role of TaHSP90A transcripts in developing phenotypic plasticity in wheat to develop heat-tolerant cultivars under the current changing climate scenario.

Comparative transcript abundance of gibberellin oxidases genes in two barley (Hordeum vulgare) genotypes with contrasting lodging resistance under different regimes of water deficit.

Barley (Hordeum vulgare ) is the world's fourth most important cereal crop, and is particularly well adapted to harsh environments. However, lodging is a major productivity constraint causing 13-65% yield losses. Gibberellic acid (GA) homeostatic genes such as HvGA20ox, HvGA3ox and HvGA2ox are responsible for changes in plant phenotype for height and internodal length that contribute towards lodging resistance. This study explored the expression of different HvGAox transcripts in two contrasting barley genotypes (5-GSBON-18, lodging resistant; and 5-GSBON-70, lodging sensitive), which were sown both under controlled (hydroponic, completely randomised factorial design) and field conditions (split-plot, completely randomised block design) with two irrigation treatments (normal with three irrigation events; and water deficit with one irrigation event). In the hydroponic experiment, expression analysis was performed on seedlings at 0, ¾, 1½, 3 and 6h after application of treatment. In the field experiment, leaf, shoot nodes and internodes were sampled. Downregulation of HvGA20ox.1 transcript and 2-fold upregulation of HvGA2ox.2 transcript were observed in 5-GSBON-18 under water deficit conditions. This genotype also showed a significant reduction in plant height (18-20%), lodging (<10%), and increased grain yield (15-18%) under stress. Utilisation of these transcripts in barley breeding has the potential to reduce plant height, lodging and increased grain yield.

Genome-wide analysis and expression divergence of protein disulfide isomerase (PDI) gene family members in chickpea (Cicer arietinum) under salt stress.

Chickpea (Cicer arietinum ) is a grain crop that is an important source of protein, vitamins, carbohydrates and minerals. It is highly sensitive to salt stress, and salt damage to cellular homeostasis and protein folding affects production. Plants have several mechanisms to prevent cellular damages under abiotic stresses, such as proteins in the endoplasmic reticulum (protein isulfide somerases (PDIs) and PDI-like proteins), which help prevent the build-up of mis-folded proteins that are damaged under abiotic stresses. In this study, we completed initial comprehensive genome-wide analysis of the chickpea PDI gene family. We found eight PDI genes are distributed on six out of eight chromosomes. Two pairs of paralogous genes were found to have segmental duplications. The phylogenetic analysis showed that the PDI s have a high degree of homology in C. arietinum, Cicer reticulatum, Lens culinaris, Phaseolus acutifolius, Pisum sativum and Oryza sativa . The gene structure analysis displayed that CaPDI1-CaPDI8 have 9-12 exons except for CaPDI5 , which has 25 exons. Subcellular localisation indicated accumulation of CaPDIs in endoplasmic reticulum. Protein-conserved motifs and domain analysis demonstrated that thioredoxin domains of PDI family is present in all CaPDIs. CaPDI proteins have strong protein-protein interaction. In silico expression analysis showed that four out of eight PDI genes (CPDI2, CaPDI6, CaPDI7 and CaPDI8 ) were expressed under salt stress. Of these, expression of CaPDI2 and CaPDI8 was the highest. This work indicated that PDI genes are involved in salt stress tolerance in chickpea and the CaPDIs may be further studied for their role of inducing salt tolerance.

Genome-wide analysis of Glutathione peroxidase (GPX) gene family in Chickpea (Cicer arietinum L.) under salinity stress.

Chickpea is the second most widely grown legume in the world. Its cultivation is highly affected by saline soils. Salt stress damages its all growth stages from germination to maturity. It has a huge genetic diversity containing adaptation loci that can help produce salt-tolerant cultivars. The glutathione peroxidase (GPX) gene family plays an important role in regulating plant response to abiotic stimuli and protects cells from oxidative damage. In current research, the role of GPX genes is studied for inducing salt tolerance in chickpea. This study identifies the GPX gene family in Cicer arietinum. In response to the NaCl stress, the gene expression profiles of CaGPX3 were examined using real-time qRT-PCR. The results of phylogenetic analysis show that CaGPX genes have an evolutionary relationship with monocots, dicots, chlorophytes, and angiosperms. Gene structure analysis showed that CaGPX3, CaGPX4, and CaGPX5 have six, CaGPX2 has five, and CaGPX1 contains 9 exons. According to the Ka and Ks analysis chickpea has one pair of duplicated genes of GPX and the duplication was tandem with negative (purifying) selection Ka < Ks (<1). In-silico gene expression analysis revealed that CaGPX3 is a salt stress-responsive gene among all other five GPX members in chickpea. The qRT-PCR results showed that the CaGPX3 gene expression was co-ordinately regulated under salt stress conditions, confirming CaGPX3's key involvement in salt tolerance.

Assessment and Exposure Analysis of Trace Metals in Different Age Groups of the Male Population in Southern Punjab, Pakistan.

In developing countries, like Pakistan, the pursuit of urbanization and economic development disrupts the delicate ecosystem, resulting in additional biogeochemical emissions of heavy metals into the human habitat and posing significant health risks. The levels of these trace elements in humans remain unknown in areas at higher risk of pollution in Pakistan. In this investigation, selected trace metals including Copper (Cu), Chromium (Cr), Lead (Pb) Cadmium (Cd), Cobalt (Co), Nickel (Ni), and Arsenic (As) were examined in human hair, urine, and nail samples of different age groups from three major cities (Muzaffargarh, Multan, and Vehari) in Punjab province, Pakistan. The results revealed that the mean concentrations (ppm) of Cr (1.1) and Cu (9.1) in hair was highest in Muzaffargarh. In urine samples, the mean concentrations (µg/L) of Co (93), As (79), Cu (69), Cr (56), Ni (49), Cd (45), and Pb (35) were highest in the Multan region, while As (34) and Cr (26) were highest in Vehari. The mean concentrations (ppm) of Ni (9.2), Cr (5.6), and Pb (2.8), in nail samples were highest in Vehari; however, Multan had the highest Cu (28) concentration (ppm). In urine samples, the concentrations of all the studied metals were within permissible limits except for As (34 µg/L) and Cr (26 µg/L) in Vehari. However, in nail samples, the concentrations of Ni in Multan (8.1 ppm), Muzaffargarh (9 ppm), Vehari (9.2 ppm), and Cd (3.69 ppm) in Muzaffargarh exceeded permissible limits. Overall, the concentrations of metals in urine, nail, and hair samples were higher in adults (39-45 age group). Cr, Cu, and Ni revealed significantly higher concentrations of metals in hair and water in Multan, whereas As in water was significantly (p < 0.001) correlated with urinary As in Multan, indicating that the exposure source was region-specific.

Prioritizing strategies for wheat biofortification: Inspiration from underutilized species.

The relationship between malnutrition and climate change is still poorly understood but a comprehensive knowledge of their interactions is needed to address the global public health agenda. Limited studies have been conducted to propose robust and economic-friendly strategies to augment the food basket with underutilized species and biofortify the staples for nutritional security. Sea-buckthorn is a known "superfood" rich in vitamin C and iron content. It is found naturally in northern hemispherical temperate Eurasia and can be utilized as a model species for genetic biofortification in cash crops like wheat. This review focuses on the impacts of climate change on inorganic (iron, zinc) and organic (vitamin C) micronutrient malnutrition employing wheat as highly domesticated crop and processed food commodity. As iron and zinc are particularly stored in the outer aleurone and endosperm layers, they are prone to processing losses. Moreover, only 5% Fe and 25% Zn are bioavailable once consumed calling to enhance the bioavailability of these micronutrients. Vitamin C converts non-available iron (Fe3+) to available form (Fe2+) and helps in the synthesis of ferritin while protecting it from degradation at the same time. Similarly, reduced phytic acid content also enhances its bioavailability. This relation urges scientists to look for a common mechanism and genes underlying biosynthesis of vitamin C and uptake of Fe/Zn to biofortify these micronutrients concurrently. The study proposes to scale up the biofortification breeding strategies by focusing on all dimensions i.e., increasing micronutrient content and boosters (vitamin C) and simultaneously reducing anti-nutritional compounds (phytic acid). Mutually, this review identified that genes from the Aldo-keto reductase family are involved both in Fe/Zn uptake and vitamin C biosynthesis and can potentially be targeted for genetic biofortification in crop plants.

Leaf wettability and leaf angle affect air-moisture deposition in wheat for self-irrigation.

BACKGROUND: Climate change and depleting water sources demand scarce natural water supplies like air moisture to be used as an irrigation water source. Wheat production is threatened by the climate variability and extremes climate events especially heat waves and drought. The present study focused to develop the wheat plant for self-irrigation through optimizing leaf architecture and surface properties for precise irrigation. METHODS: Thirty-four genotypes were selected from 1796 genotypes with all combinations of leaf angle and leaf rolling. These genotypes were characterized for morpho-physiological traits and soil moisture content at stem-elongation and booting stages. Further, a core set of ten genotypes was evaluated for stem flow efficiency and leaf wettability. RESULTS: Biplot, heat map, and correlation analysis indicated wide diversity and traits association. The environmental parameters indicated substantial amount of air moisture (> 60% relative humidity) at the critical wheat growth stages. Leaf angle showed negative association with leaf rolling, physiological and yield traits, adaxial and abaxial contact angle while leaf angle showed positive association with the stem flow water. The wettability and air moisture harvesting indicated that the genotypes (coded as 1, 7, and 18) having semi-erect to erect leaf angle, spiral rolling, and hydrophilic leaf surface (<90o) with contact angle hysteresis less than 10o had higher soil moisture content (6-8%) and moisture harvesting efficiency (3.5 ml). CONCLUSIONS: These findings can provide the basis to develop self-irrigating, drought-tolerant wheat cultivars as an adaptation to climate change.

GhCDNC and GhCYP706B1 genes mediate gossypol biosynthesis in upland cotton.

BACKGROUND: In cotton the identification and characterization of natural defense is a cost-effective, sustainable, and environment-friendly strategy to combat cotton pests. The secondary metabolites traits in cotton plant i.e., toxic gossypol glands play significant role for development and self-defense mechanism. To utilize gossypol in breeding implements, the understanding of gossypol initiation biosynthesis genes has vital importance at reproductive organ development stages. METHODS: Cotton germplasm of 100 genotypes screened visually based on gossypol glandedness and a core set of ten genotypes was developed. Further three genotypes FH-330 (high glanding), F-280 (low glanding) and IRMA-197 (glandless) were used for determining the transcript abundance of twelve gossypol biosynthesis genes. RESULTS: Out of 100, germplasm categorized as (76) high glanding, (22) medium glanding), one genotype for each (low glanding) and (glandless) category. Real-time qPCR analysis revealed varied expression patterns among selected three genotypes. Out of twelve, three genes CYP706B1, CDNC and 2ODD-1 had strong expression levels in all tested tissues in high glanded genotype, while, slight or no expression of these genes was recorded in low glanding and glandless genotype, respectively. The shell of developing boll (10, 20, 30 DPA), and developing embryo (20, 30 DPA) showed substantially medium to maximum expression, respectively while high to medium expression was recorded in sepals and leaf tissue. CONCLUSIONS: Our study demonstrated that CYP706B1, CDNC and 2ODD-1 are the most promising genes involved in gossypol biosynthesis. Developing boll shell, developing embryo, leaf and sepal also have significant ability to synthesize gossypol. This will provide scientists a way to manipulate gossypol contents in economically important organs of cotton plant for targeted breeding.

Wetting mechanism and morphological adaptation; leaf rolling enhancing atmospheric water acquisition in wheat crop-a review.

Several plant species such as grasses are dominant in many habitats including arid and semi-arid areas. These species survive in these regions by developing exclusive structures, which helps in the collection of atmospheric water. Before the collected water evaporates, these structures have unique canopy structure for water transportation that plays an equivalent share in the fog-harvesting mechanism. In this review, the atmospheric gaseous water harvesting mechanisms and their affinity of measurements were discussed. Morphological adaptations and their role in the capturing of atmospheric gaseous water of various species were also discussed. The key factor for the water collection and its conduction in the wheat plant is the information of contact angle hysteresis. In wheat, leaf rolling and its association with wetting property help the plant in water retention. Morphological adaptations, i.e., leaf erectness, grooves, and prickle hairs, also help in the collection and acquisition of water droplets by stem flows in directional guide toward the base of the plant and allow its rapid uptake. Morphological adaptation strengthens the harvesting mechanism by preventing the loss of water through shattering. Thus, wheat canopy architecture can be modified to harvest the atmospheric water and directional movement of water towards the root zone for self-irrigation. Moreover, these morphological adaptations are also linked with drought avoidance and corresponding physiological processes to resist water stress. The combination of these traits together with water use efficiency in wheat contributes to a highly efficient atmospheric water harvesting system that enables the wheat plants to reduce the cost of production. It also increases the yielding potential of the crop in arid and semi-arid environments. Further investigating the ecophysiology and molecular pathways of these morphological adaptations in wheat may have significant applications in varying climatic scenarios.

A Short Review on Key Role of Plants and their Extracts in Boosting up Immune Response to Combat COVID-19.

In the wake of the recent global pandemic of COVID-19, there has been increasing concern among the general public to improve their immune system. The causative agent of COVID-19 is SARS CoV-2, similar to its relative viruses SARS Cov-1 and MERS. Although vaccines have been developed now and are in public use, still the role of a healthy immune system is crucial as new strains of the virus are being discovered and along with emergence of new strains, short and long term side effects of vaccine cannot be excluded. Plants have been used in herbal medicines and many chemicals since ancient times and extracts in them are found to boost the immune system. Therefore, a consciously maintained diet consisting of plant-based immunity boosters is the need of the hour. Plants are known to provide many chemicals, natural therapeutics and vitamins which naturally enhance our immune system. Recently, vaccination research is also being carried out in transgenic plants. In this review, main focus has been put on highlighting some of the key players within the Kingdom Plantae, which, when taken up in regular diet, can significantly boost our immune system capacity and thus helps the human body to combat this deadly virus more efficiently.

Leaf prickle hairs and longitudinal grooves help wheat plants capture air moisture as a water-smart strategy for a changing climate.

MAIN CONCLUSION: The leaf features like trichome density, gradient grooves, and leaf wettability determine the efficiency to capture air moisture for self-irrigation in the wheat plant. Plants in water-scarce environments evolved to capture air moisture for their water needs either directly or indirectly. Structural features like cones, hairs, and grooves assist water capture. The morphology of crops such as wheat can promote self-irrigation under drought. To examine this further, 34 wheat genotypes were characterized for leaf traits in near optimal conditions in the field using a randomized complete block design with 3 replications. An association was found between morphological and physiological traits and yield using simple correlation plots. A core set of nine genotypes was subsequently evaluated for moisture harvesting ability and leaf wettability. Results showed that variation among genotypes exists for fog harvesting ability attributed to structural leaf features. Physiological traits, especially photosynthesis and water use efficiency, were positively associated with yield, negatively correlated with soil moisture at booting, and positively correlated with soil moisture at anthesis. The genotypes with deep to medium leaf grooves and dense hairs on the edges and adaxial surfaces (genotypes 7 and 18) captured the most moisture. This was a function of higher water drop rolling efficiency resulting from lower contact angle hysteresis. These results can be exploited to develop more heat and drought-tolerant crops.

Development and Exploitation of KASP Assays for Genes Underpinning Drought Tolerance Among Wheat Cultivars From Pakistan.

High-throughput genotyping for functional markers offers an excellent opportunity to effectively practice marker-assisted selection (MAS) while breeding cultivars. We developed kompetitive allele-specific PCR (KASP) assays for genes conferring drought tolerance in common wheat (Triticum aestivum L.). In total, 11 KASP assays developed in this study and five already reported assays were used for their application in wheat breeding. We investigated alleles at 16 loci associated with drought tolerance among 153 Pakistani hexaploid wheat cultivars released during 1953-2016; 28 diploid wheat accessions (16 for AA and 12 for BB) and 19 tetraploid wheat (AABB) were used to study the evolutionary history of the studied genes. Superior allelic variations of the studied genes were significantly associated with higher grain yield. Favored haplotypes of TaSnRK2.3-1A, TaSnRK2.3-1B, TaSnRK2.9-5A, TaSAP-7B, and TaLTPs-1A predominated in Pakistani wheat germplasm indicating unconscious pyramiding and selection pressure on favorable haplotypes during selection breeding. TaSnRK2.8-5A, TaDreb-B1, 1-feh w3, TaPPH-7A, TaMOC-7A, and TaPARG-2A had moderate to low frequencies of favorable haplotype among Pakistani wheat germplasm pointing toward introgression of favorable haplotypes by deploying functional markers in marker-assisted breeding. The KASP assays were compared with gel-based markers for reliability and phenotypically validated among 62 Pakistani wheat cultivars. Association analyses showed that the favorable allelic variations were significantly associated with grain yield-contributing traits. The developed molecular marker toolkit of the genes can be instrumental for the wheat breeding in Pakistan.

Inoculation with the endophyte Piriformospora indica significantly affects mechanisms involved in osmotic stress in rice.

BACKGROUND: Rice is a drought susceptible crop. A symbiotic association between rice and mycorrhizal fungi could effectively protect the plant against sudden or frequent episodes of drought. Due to its extensive network of hyphae, the endophyte is able to deeply explore the soil and transfer water and minerals to the plant, some of them playing an important role in mitigating the effects of drought stress. Moreover, the endophyte could modify the expression of drought responsive genes and regulate antioxidants. RESULTS: Three rice genotypes, WC-297 (drought tolerant), Caawa (moderately drought tolerant) and IR-64 (drought susceptible) were inoculated with Piriformospora indica (P. indica), a dynamic endophyte. After 20 days of co-cultivation with the fungus, rice seedlings were subjected to 15% polyethylene glycol-6000 induced osmotic stress. P. indica improved the growth of rice seedlings. It alleviated the destructive effects of the applied osmotic stress. This symbiotic association increased seedling biomass, the uptake of phosphorus and zinc, which are functional elements for rice growth under drought stress. It boosted the chlorophyll fluorescence, increased the production of proline and improved the total antioxidant capacity in leaves. The association with the endophyte also up regulated the activity of the Pyrroline-5-carboxylate synthase (P5CS), which is critical for the synthesis of proline. CONCLUSION: A mycorrhizal association between P. indica and rice seedlings provided a multifaceted protection to rice plants under osmotic stress (- 0.295 MPa).